Retno Widowati, Universitas Nasional, Indonesia
Sri Handayani, Universitas Nasional, Indonesia
Suprihatin, Universitas Nasional, Indonesia
Indra Lesmana Rahayu, Universitas Nasional, Indonesia
Nyoman Yudi Antara, Universitas Nasional, Indonesia
The research aimed to find out the ability of methanol extract of G. salicornia, H. gracilis, H. macroloba, and H. asperi from Indonesia as antibacterial in inhibiting E. coli (ATCC 8739), S. aureus (ATCC 6539), and P. aeruginosa (ATCC 9027). The research design is quasi experimental by using statistic group comparison. The test method for antibacterial activity is well diffusion which refers to CLSI document M02-A11. The antibacterial activity test showed the highest inhibitory zone to E. coli, only in methanol extract of G. salicornia 20%, the highest inhibitory zone to S. aureus is in methanol extract of H. asperi 20%, and the highest inhibitory zone to P. aeruginosa is in methanol extract of H. gracilis 20%. The differences in the effect of macro algae methanol extract which tested at the same concentration on S. aureus showed that significant effect on methanol extract of H. asperi and G. salicornia at concentration 20% (p ≤ 0,05) and to P. aeruginosa did not showed significant difference of influence, and comparison of methanol macro algae extract effect at same concentration between S. aureus and P. aeruginosa did not show significant effect difference (p> 0,05).
Antibacterial; Macro algae; Methanol extract
[1] Suparmi and A. Sahri, "Mengenal potensi rumput laut : Kajian pemanfaatan sumber daya rumput laut dari aspek industri dan kesehatan", Jurnal Majalah Ilmiah Sultan Agung., vol. 44, (2009): 95-116.
[2] S. Handayani, T. M. Setia, S. E. Rahayu, "Pengenalan makroalgae Indonesia", Dian Rakyat, Jakarta, (2014).
[3] S. K. Kim, "Marine proteins and peptides biological activities and applications", Blackwell Publisher, United Kingdom, (2013).
[4] S. Handayani and R. Widowati, "Inventarisasi dan identifikasi spesies makroalgae di pulau Tidung Kepulauan Seribu dalam upaya menggali sumber hayati potensial", Universitas Nasional, Jakarta, (2016).
[5] R. Alghazeer, F. Whida, E. Abduelrhman, F. Gammoudi and S. Azwai, “Screening of antibacterial activity in marine green, red and brown macro algae from the western coast of Libya”, Natural Science., vol. 5, (2013): 7-14.
[6] P. Arunachalam, R. Uthandakalai and R. Rajsmaill, "Evaluation of antibacterial activity of some selected green seaweed extracts from Muttam coastal areas, Kanyakumari, Tamil Nadu, India", Journal of Coastal Life Medicine., vol. 2, (2014): 112-115.
[7] Al-Saif SS, Abdel-Raouf N, El-Wazanani HA and Aref IA, "Antibacterial substances from marine algae isolated from Jeddah coast of Red sea, Saudi Arabia", Saudi Journal of Biological Sciences., vol. 21, (2014): 57-64.
[8] A. Ismail, L. Ktari, M. Ahmed, H. Bolhuis, A. Bouddabous, L. J. Stal, M. S. Cretoiu and M. E. Bour, "Antimicrobial activities of bacteria associated with the brown algae Padina pavonica", Frontiers in Microbiology., vol. 7, no. 72016, (2016): 1-13.
[9] S. Khoiriyah, A. Hanapi and A. G. Fasya, "Uji fitokimia dan aktivitas antibakteri fraksi etil asetat, kloroform dan petroleum eter ekstrak metanol algae coklat Sargassum vulgare dari pantai kapong pamekasan Madura", ALCHEMY., vol. 3, (2014): 133-144.
[10] N. Karthick, M. A. Fathimal, K. Ramesh, H. Sridhar, M. Natrajan, V. V. Divya, M. Umanitha and S. Umamaheswari, "Screening of phytochemicals and antimicrobial activity of Caulerpa scalpelliformis collected from Manapad Coast, Tuticorin District, Tamilnadu, South India", Journal of Coastal Life Medicine., vol. 2, (2014): 107-111.
[11] R. Paramsivam, S. Sudevan, S. Sundar and V. Ramasamy, "Study on metabolic compounds of Gracilaria salicornia against anti-inflammatory activity", International Journal of Current Microbiology and Applied Sciences., vol. 5, (2016): 202-211.
[12] R. Alghazeer, A. Elmansori, M. Sidati, F. Gammoudi, S. Azwai, H. Naas, A. Garbaj and I. Eldaghayes, “In vitro antibacterial activity of flavonoid extracts of two selected Libyan algae against multi-drug resistant bacteria isolated from food products”, Journal of Bioscience and Medicines., vol. 5, (2017); 26-48.
[13] Z. Demirel, F. F. Yilmaz-Koz, U. N. Karabay-Yavasoglu, G. Ozdemir and A. Sukatar, "Antimicrobial and antioxidant activity of brown algae from the Aegean Sea", Journal of the Serbian Chemical Society, vol. 74, (2009); 619-628.
[14] Prasetya dan E. Inoriah, "Pengelolaan budidaya tanaman obat-obatan (bahan simplicia)", Badan Penerbitan Fakultas Pertanian UNIB, Bengkulu, (2013).
[15] CLSI, "Performance standards for antimicrobial disk susceptibility tests; Approved standard—Eleventh edition. In CLSI document M02-A11", Wayne, PA, USA, (2012).
[16] CLSI, "Performance standards for antimicrobial susceptibility testing; Twenty-first informational supplement. In CLSI document M100-S21", Wayne, PA, USA, (2011).
[17] H. J. Seltman, "Experimental design and analysis", Carnegie Mellon University, USA, (2015).
[18] C. Govindasamy, S. Narayani, M. Arulpriya, P. Ruban, K. Anantharaj and R. Srinivasan, "In vitro antimicrobial activities of seaweed extracts against human pathogens", Journal of Pharmacy Research., vol. 4, (2011); 2076-2077.
[19] P. Mamahit, J. Wuisan, P. S. Anindita, "Efektivitas ekstrak daun jambu mawar (Syzigium jambos L. Alston) menghambat pertumbuhan Streptococcus mutans secara in vitro", Pharmacon Jurnal Ilmiah Farmasi, vol. 5, (2016); 53-58.
[20] S. J. Cavalieri, "Manual of antimicrobial susceptibility testing", American Society for Microbiology, USA, (2005).
[21] H. Inayati, "Potensi antibakteri ekstrak daun kedondong bangkok (Spondias dulcis Forst.)" Institut Pertanian Bogor, Bogor, (2007).
[22] Nurfadilah, "Uji bioaktifitas antibakteri ekstrak dan fraksi lamun dari Kepulauan Spermonde, Kota Makassar", Universitas Hasanuddin, Makassar, (2013).
[23] G. F. Brooks, K. C. Carroll, J. S. Butel, S. A. Morse and T. A. Meitzner, "Jawetz, Melnick, & Adelberg’s Medical Microbiology", The McGraw-Hill Companies, Inc, (2013).
[24] S. Madduluri, K. B. Rao, B. Sitaram, "In vitro evaluation of antibacterial activity of five indigenous plants extract against five bacterial pathogens of human", International Journal of Pharmacy and Pharmaceutical Sciences., vol. 5, (2013); 679-684.
[25] M. Poeloengan and Praptiwi, "Uji aktivitas antibakteri ekstrak kulit buah manggis (Gardnia mangostana Linn)", Media Litbang Kesehatan., vol. 20, (2010); 65-69.
[26] Y. Fatisa, "Daya antibakteri estrak kulit dan biji buah pulasan (Nephelium mutabile) terhadap Staphylococcus aureus dan Escherichia coli secara in vitro", Jurnal Peternakan., vol. 10, (2013); 31-38.
[27] Silvikasari, "Aktivitas antibakteri ekstrak kasar flavonoid daun gambir (Uncaria gambir Roxb)", Institut Pertanian Bogor, Bogor, (2011).
APA:
Widowati, R., Handayani, S., Suprihatin, Rahayu, I. L., & Antara, N. Y. (2019). Antibacterial Activity of Methanol Extract of Gracilaria Salicornia, Halimeda Gracilis, Halimeda Macroloba, and Hypnea Asperi From Indonesia. International Journal of Advanced Science and Technology (IJAST), ISSN: 2005-4238(Print); 2207-6360 (Online), NADIA, 129, 67-80. doi: 10.33832/ijast.2019.129.06.
MLA:
Widowati, Retno, et al. “Antibacterial Activity of Methanol Extract of Gracilaria Salicornia, Halimeda Gracilis, Halimeda Macroloba, and Hypnea Asperi From Indonesia.” International Journal of Advanced Science and Technology, ISSN: 2005-4238(Print); 2207-6360 (Online), NADIA, vol. 129, 2019, pp. 67-80. IJAST, http://article.nadiapub.com/IJAST/Vol129/6.html.
IEEE:
[1] R. Widowati, S. Handayani, Suprihatin, I. L. Rahayu, and N. Y. Antara, “Antibacterial Activity of Methanol Extract of Gracilaria Salicornia, Halimeda Gracilis, Halimeda Macroloba, and Hypnea Asperi From Indonesia.” International Journal of Advanced Science and Technology (IJAST), ISSN: 2005-4238(Print); 2207-6360 (Online), NADIA, vol. 129, pp. 67-80, Aug. 2019.
IJAST aims to facilitate and support research related to advanced science and technology and the applications. Our Journal provides a chance for academic and industry professionals to discuss recent progress in the areas of advanced science and technology.
